![]() We demonstrate the potential of this new imaging platform by quantitatively imaging near-infrared (NIR) Förster resonance energy transfer (FRET) both in vitro and in vivo. ![]() The system makes use of three digital micromirror device (DMD)-based spatial light modulators (SLMs) to generate spatial optical bases and reconstruct N by N images over 16 spectral channels with a time-resolved capability ( ∼40 ps temporal resolution) using fewer than N 2 optical measurements. An imaging platform based on time-resolved structured light and hyperspectral single-pixel detection has been developed to perform quantitative macroscopic fluorescence lifetime imaging (MFLI) over a large field of view (FOV) and multiple spectral bands simultaneously. Spectrally resolved fluorescence lifetime imaging 1, 2, 3 and spatial multiplexing 1, 4, 5 have offered information content and collection-efficiency boosts in microscopy, but efficient implementations for macroscopic applications are still lacking.
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